METHOD FOR PREDICTION OF ANDROGENIC ALOPECIA IN MALES Russian patent published in 2020 - IPC C12Q1/68 G01N33/74 G01N33/82 G01N33/84 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to dermatology and cosmetology, and can be used for prediction of androgenic alopecia in males by examining genetic predisposition to development of this disease and determining pathogenetically significant non-genetic indicators. A marker of the genetic predisposition is the single nucleotide polymorphism rs929626 in the EBF1 gene. Non-genetic blood plasma values are concentrations of total testosterone, dihydrotestosterone, copper, selenium, vitamin D and glucose. That is used to assess the probability of alopecia onset and progression with achievement of a certain stage of the disease according to Norwood-Hamilton classification by values of classification functions of linear discriminant analysis: F₀=A₁×2.5385+A₂×0.1143+A₃×0.0053+A₄×1.3781+A₅×23.6958+A₆×0.3191+A₇×16.0517-74.1424; F₂=A₁×7.1999+A₂×-0.0708+A₃×0.0058+A₄×0.4550+A₅×19.264+A₆×0.1182+A₇×18.0666-65.3727; F₃=A₁×3.9119+A₂×0.0425+A₃×0.0061+A₄×0.7940+A₅×21.1388+A₆×0.1906+A₇×15.8956-57.6115; F₄=A₁×3.9881+A₂×0.0874+A₃×0.0083+A₄×1.2447+A₅×15.9065+A₆×0.1867+A₇×16.8561-69.4355; F₅=A₁×3.9716+A₂×0.1348+A₃×0.0052+A₄×0.8559+A₅×25.3735+A₆×0.1884+A₇×13.2247-54.6796, where variables are: A₁ – number of genotype of mononucleotide polymorphism rs929626 of allele variant G associated with predisposition to alopecia, namely AA=0, GA=1, GG=2; A₂ is blood plasma total testosterone concentration, nmol/l; A₃ is concentration of dihydrotetosterone in blood plasma, pg/ml; A₄ is copper concentration in blood plasma, mcmol/l; A₅ is selenium concentration in blood plasma, mcg/l; A₆ is concentration of vitamin D in blood plasma, ng/ml; A₇ is concentration of glucose in blood plasma, mmol/l. Thereafter, the onset and development of androgenic alopecia is predicted from the maximum value of the classification equation: F₀ absence of risk of alopecia; F₂, F₃, F₄ and F₅ – II, III, IV and V stage of alopecia, respectively.

EFFECT: method provides prediction of androgenic alopecia in males due to integral assessment of the most informative markers of genetic predisposition and determination of a number of biochemical blood indices (hormones, microelements and vitamins) significant for progress and regress of the given disease.

1 cl, 6 dwg, 1 tbl, 2 ex