METHOD FOR CULTIVATION OF MICROALGAE CHROMOCHLORIS ZOFINGIENSIS FOR PRODUCING LIPIDS AND CAROTENOIDS Russian patent published in 2020 - IPC C12N1/12 C12P23/00 C12R1/89 

FIELD: biotechnologies.

SUBSTANCE: invention relates to the biotechnology. Method of cultivation of microalgae Chromochloris zofingiensis involves cultivation of microalgae Chromochloris zofingiensis on "green stage" on nutrient medium of MBBM for 17 days at temperature 22–23 °C with preset lighting and air blowdown rate of culture. Culture is transferred to "red stage" by culture cultivation with nutrient medium with reduced content of nitrogen and phosphorus and grown for 15 days with duration of light period of 24 hours, at a given temperature and given illumination. Nutrient medium for "green" and "red" cultivation stage contains CO(NH₂)_2, MgSO₄⋅7H₂O, NaCl, K₂HPO₄⋅3H₂O, KH₂PO₄, Na₂-EDTA, KOH, H₃BO₃, ZnSO₄⋅7H₂O, MnCl₂⋅4H₂O, Na₂MoO₄⋅2H₂O, CuSO₄⋅5H₂O, Co(NO₃)₂⋅6H₂O, FeSO₄⋅7H₂O, CaCl_2, H₂SO_{4 end} and distilled water at specified content of components.

EFFECT: invention increases output of lipids and ketocaratinoids.

3 cl, 3 dwg, 5 tbl, 1 ex