METHOD FOR PREDICTION OF PURULENT PYELONEPHRITIS DEVELOPMENT Russian patent published in 2020 - IPC G01N33/68 G01N33/577 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to urology and hematology, and can be used for prediction of purulent pyelonephritis by examining venous blood. Blood sampling is performed at admission to hospital. Freshly collected blood samples are placed immediately on a heating pad with ice, centrifuged during day at 1400g for 15 minutes at room temperature, sampling supernatant for examination. Serial dilutions of dissolved ET-SROCK are prepared in a culture medium to 0.625 fmol/ml using a zero standard. Immunoassay kit is used to quantify human endothelium 1–21 in serum, plasma, urine, saliva, and supernatant cell cultures. 50 mcl standards, samples and controls STD/SAMPLE/CTRL are introduced into the respective cells in duplicate, the cavity blank is left empty. One adds 200 mcl of detecting AB antibodies to each well except for the blend and mixed. Strips are covered with film and incubated for 16–24 hours at room temperature 18–26 °C. During the examination, the film is removed from the strips, the contents of the cells are completely removed and washed 5 times with 300 mcl each with a diluted buffer for flushing WASHBUF. Liquid residue is removed in cells after last washing by turning strips onto filter paper. One adds 200 mcl of enzyme conjugate CONJ into all cells, covers the strips with a film and incubates for 1 hour at room temperature 18–26 °C. Film is removed from the strips, the contents of the cells are completely removed and washed 5 times with 300 mcl each with diluted WASHBUF flushing buffer, removed liquid residue in cells after the last washing is turned over onto strips on filtering paper. Substrate 200 is introduced into all cells, incubated for 30 minutes at room temperature 18–26 °C in the dark. 50 mcl STOP stop solution is added to all cells, the cell contents are mixed. Optical density of the cells is immediately considered at 450 nm with a comparison wavelength of 630 nm. Value of optical density - OD of the blank is subtracted from the values of the other cells of the other cells, a calibration curve is plotted, results obtained using this method using 4PL algorithm are evaluated. Level of endothelin-1 is determined, and if value 1.20 fmol/ml and more, purulent pyelonephritis is predicted.

EFFECT: method provides prediction of development of purulent pyelonephritis, allowing to determine the transition of serous stage of pyelonephritis, purulent, by assessing the state and changes on the side of renal vessels, namely vascular endothelium injury accompanied by progression of the inflammatory process.

1 cl, 2 dwg, 2 ex