FIELD: biotechnology.
SUBSTANCE: developed set of oligonucleotide primers and fluorescence-labeled probe for identification of West Nile virus lineage 4 by polymerase chain reaction with reverse transcription and hybridization-fluorescence allowance for results, complementary to West Nile virus lineage 4 polyprotein gene fragments, having activity of forward and reverse primers, and a probe designed as "molecular beacon", providing fluorescent detection of amplification product with size of 147 base pairs in real time.
EFFECT: invention enables high-specific detection of West Nile virus lineage 4 in biological material in a short period of time.
1 cl, 3 ex, 1 tbl, 1 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS 1 GENOTYPE | 2019 |
|
RU2715625C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS GENOTYPE 2 | 2019 |
|
RU2715617C1 |
| BACTERIAL STRAIN ESCHERICHIA COLI JM 109 4-162, PRODUCER OF RECOMBINANT PLASMID PWNV4PROTC, CARRYING SEQUENCE OF POLYPROTEIN GENE SEGMENT OF WEST NILE VIRUS GENOTYPE 4 ENCODING CAPSID PROTEIN | 2020 |
|
RU2739333C1 |
| ESCHERICHIA COLI JM 109 1-430 BACTERIAL STRAIN - PRODUCER OF THE RECOMBINANT PWNV1PROTC PLASMID, WHICH CARRIES THE SEQUENCE OF THE 5'-UNTRANSLATED AREA AND A SECTION OF THE WEST NILE VIRUS 1 POLYPROTEIN GENE OF THE GENOTYPE ENCODING THE CAPSID PROTEIN | 2020 |
|
RU2744095C1 |
| ESCHERICHIA COLI JM 109 2-428 BACTERIAL STRAIN - PRODUCER OF THE RECOMBINANT PWNV2PROTC PLASMID, WHICH CARRIES THE SEQUENCE OF THE 5'-UNTRANSLATED AREA AND A SECTION OF THE WEST NILE VIRUS 2 POLYPROTEIN GENE OF THE GENOTYPE ENCODING THE CAPSID PROTEIN | 2020 |
|
RU2744096C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR IDENTIFICATION OF TICK-BORNE ENCEPHALITIS VIRUS, WEST NILE FEVER, BORRELIA AND RICKETTSIA BY REAL TIME MULTIPLEX PCR | 2016 |
|
RU2629604C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY MARKED PROBE IN TAQMAN FORMAT FOR DETECTION OF VIRUSES OF FLAVIVIRUS GENUS BY REAL-TIME PCR | 2022 |
|
RU2808520C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
|
RU2818960C1 |
| REAGENT SET FOR DETECTING RNA OF SARS-COV-2 VIRUS, CAUSATIVE AGENT OF NEW CORONAVIRUS DISEASE COVID-2019, BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION IN REAL TIME | 2020 |
|
RU2732608C1 |
| METHOD FOR DETECTING RNA OF AN ARTERITIS VIRUS AGENT IN HORSES | 2018 |
|
RU2700481C1 |
