FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and genetic engineering and concerns a plasmid for transfection into human cells to detect their epithelial state with respect to an epithelial-mesenchymal transformation process involving a segment with a nucleotide sequence SEQ ID NO:1 of a marker epithelial gene promoter, in form of human epithelial cadherin and at least one sequence of TurboGFP fluorescent protein, wherein the region of the nucleotide sequence of the fluorescent protein is under the control of the promoter of the epithelial status marker gene. Invention can be used in high-efficiency search of medical products, and also for studying of factors influencing efficiency of metastasis.
EFFECT: disclosed is a plasmid for detecting an epithelial state of a human cell.
1 cl, 3 dwg
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