METHOD OF DETERMINING STRUCTURAL STATE OF ERYTHROCYTE MEMBRANE Russian patent published in 2014 - IPC G01N33/50 

FIELD: biotechnology.

SUBSTANCE: method is implemented as follows. The erythrocytes from the blood sample are washed with physiological saline, then centrifuged, then extracted with chloroform-methanol mixture in the ratio 1: 2, at that shaken periodically, and the ratio of sample: extractant is 0.1: 2.0. Then the extract of erythrocytes and standards of phosphatidylcholine and phosphatidylethanolamine are applied to the chromatographic plate with the sorbent Sorbfil PTSH-AF-A-UF, which is placed in a chromatography chamber with eluent of composition - chloroform:methanol:glacial acetic acid:water taken in a ratio of 60:50:1:4. After 10-15 minutes the chromatographic plate is removed, dried and then developed in iodine vapour. The phosphatidylcholine and phosphatidylethanolamine in the sample are identified according to the path length of the sample spots compared to the path length of standard spots. In case of exceeding the spot size of phosphatidylethanolamine compared to the spot of phosphatidylcholine the damage of the structure of the erythrocyte membrane is determined.

EFFECT: method enables to determine the damage of the erythrocyte membrane structure, which enables to improve the diagnosis of the disease at the cellular level and to carry out monitoring of the efficiency of treatment.

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