CRISPR-CAS SYSTEMS AND METHODS FOR CHANGING THE EXPRESSION OF GENE PRODUCTS Russian patent published in 2023 - IPC C12N15/10 C12N15/63 C12N15/85 C12N15/90 C12N9/22 

FIELD: biotechnology.

SUBSTANCE: invention relates to a designed CRISPR-Cas system containing Cas9 protein or one or more nucleic acid molecules encoding Cas9 protein, and a chimeric RNA of the CRISPR-Cas system or one or more nucleic acid molecules encoding this chimeric RNA. Cas9 is fused to at least one nuclear localization signal (NLS) where the chimeric RNA is capable of forming a CRISPR complex with the Cas9 protein and directing the Cas9 protein to a target sequence in a eukaryotic cell.

EFFECT: invention is effective for modifying an eukaryotic cell that is not a human germline cell.

1 cl, 22 dwg, 4 tbl, 7 ex