FIELD: medicine.
SUBSTANCE: invention refers to medicine, namely to biotechnology, and can be used for expansion of mesenchymal cells of donor bone marrow. That is ensured by determining the amount of aldehyde dehydrogenase-positive mesenchymal cells and normalizing their density per unit area of culture flasks, where the seeding density is more than 500 aldehyde dehydrogenase-positive cells per cm2.
EFFECT: using the given method enables increasing growth rates of mesenchymal cell culture with doubling the number of cells and achieving confluent monolayer cells.
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