FIELD: medicine.
SUBSTANCE: invention claims method of osteogenic potential assessment for mesenchymal stromal cells (MSC). Method is based on optic density measurement of MSC cultures within twice increasing cell concentration range, further cell cultivation in osteogenic induction medium and calcium deposit colouring by silver nitrate.
EFFECT: defining optimal conditions of MSC function depending on tissue source type of MSC, MSC cell density, multiplication factor, MSC subculture duration in vitro, serum growth factor concentration in microenvironment, concomitant pathology character.
4 ex, 5 tbl, 4 cl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR ASSESSING OSTEOGENETIC POTENTIAL OF MESENCHYMAL STROMAL CELLS | 2016 |
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