METHOD FOR DETECTING PATHOLOGICAL CHANGES IN STRUCTURAL ELEMENTS OF ERYTHROCYTE MEMBRANES Russian patent published in 2020 - IPC G01N33/49 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to hematology, and can be used for detecting pathological changes in structural elements of erythrocyte membranes (EM). For this purpose, using an atomic power microscope, an image of the EM surface is obtained and decomposed into images of surfaces of three scales P₁, P₂, P₃. Scale P₁ corresponds to flickering parameters EM. Scale P₂ corresponds to the parameters of the spectrin matrix EM. Scale P₃ corresponds to parameters of protein clusters EM. Heights of detected topological nanostructures, their average values and their mean square deviations are measured. Then probability of deviation of height values of nanostructures P₁, P₂, P₃ from normal values in the control is determined. At the same time deviation of value P₁ more than 30 % for P₁ indicates deviation of value of flickering amplitude. Value P₂ more than 30 % for P₂ testifies to deviation of physical characteristics of spectrinum matrix. P₃ value more than 30 % for P₃ indicates clustering of intermembrane proteins.

EFFECT: invention makes it possible to quantify the degree of the pathological factor effect on the erythrocyte membrane state.

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