METHOD FOR DETERMINING RHEOLOGICAL BLOOD PROPERTIES Russian patent published in 2020 - IPC G01N33/49 G01N15/10 

FIELD: measurement; medicine.

SUBSTANCE: invention relates to a method for determining blood rheological properties. Method involves assessment of erythrocyte deformability under external force pressure without disturbing cell integrity. Cell sample is analyzed in native medium using atomic force spectroscopy by stepwise deflection of each erythrocyte membrane by indenter to depth h=1000–1200 nm, with pitch Δh=4–5 nm, fixing the F_i(h_i)_experim dependence, where F_i is the impact force, h_i is the deflection depth, i=1, … N, N is number of registration points, minimum value of deflection h_Hz is determined, at which dependence of force of action on depth of deflection ceases to comply with Hertz formula

where F_Hertz is the exposure force, E is Young's modulus of the erythrocyte membrane, R is the radius of the indenter, h is the deflection depth, the probability P of the deviation h_Hz is determined from the reference values h_Hzcontr=760±280 nm, corresponding to physiological conditions of erythrocytes. Value P more than 20 % testifies to deterioration of rheological properties of blood.

EFFECT: disclosed method enables to evaluate the elastic properties of specific erythrocyte membranes and can be used to diagnose various pathological rheological processes in the blood.

1 cl, 5 dwg, 1 tbl