FIELD: measurement; medicine.
SUBSTANCE: invention relates to a method for determining blood rheological properties. Method involves assessment of erythrocyte deformability under external force pressure without disturbing cell integrity. Cell sample is analyzed in native medium using atomic force spectroscopy by stepwise deflection of each erythrocyte membrane by indenter to depth h=1000–1200 nm, with pitch Δh=4–5 nm, fixing the Fi(hi)experim dependence, where Fi is the impact force, hi is the deflection depth, i=1, … N, N is number of registration points, minimum value of deflection hHz is determined, at which dependence of force of action on depth of deflection ceases to comply with Hertz formula
where FHertz is the exposure force, E is Young's modulus of the erythrocyte membrane, R is the radius of the indenter, h is the deflection depth, the probability P of the deviation hHz is determined from the reference values hHzcontr=760±280 nm, corresponding to physiological conditions of erythrocytes. Value P more than 20 % testifies to deterioration of rheological properties of blood.
EFFECT: disclosed method enables to evaluate the elastic properties of specific erythrocyte membranes and can be used to diagnose various pathological rheological processes in the blood.
1 cl, 5 dwg, 1 tbl
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Authors
Dates
2020-07-09—Published
2020-03-18—Filed