FIELD: biotechnology.
SUBSTANCE: described is a method for identifying potential agents, comprising: (a) providing a library of potential agents, in which each potential agent is attached to a unique nucleic acid marker, having a marker sequence, wherein the potential agents are selected from a group consisting of proteins, nucleic acids, cells and small molecules, where the small molecules are selected from a group, consisting of potential enzyme inhibitors, potential antibiotics, potential antiviral agents, potential pesticides, potential hormones, potential cellular signaling activators, potential inhibitors of cell signaling and potential activators of enzymes; (b) attaching a library of potential agents to a solid support comprising primers of nucleic acids, by hybridization of nucleic acid markers with nucleic acid primers to form an array of potential agents; (c) bringing the array of potential agents into contact with a screening agent, wherein one or more of the array potential agents reacts with a screening agent, where the screening agent reacts with one or more potential agents by binding to one or more potential agents or blocking binding between the potential agent and the analyte, having an affinity to a potential agent; (d) examining an array during or after contacting the array with a screening agent to establish that at least one potential agent in the array, reacts with a screening agent, wherein the array examination involves detecting a screening agent which is associated with one or more potential agents, where the screening agent is luminescent, and detection is detection of luminescence from array; (e) sequencing the nucleic acid markers in the array to determine the marker sequence, which is attached to each of the potential agents; and (f) identifying at least one potential agent in the array that reacts with the screening agent based on the determined marker sequence.
EFFECT: invention extends range of methods for identifying potential agents.
18 cl, 12 dwg, 4 ex
