METHOD AND COMPOSITION FOR ANALYZING CELLULAR COMPONENTS Russian patent published in 2020 - IPC C12Q1/6806 

FIELD: biotechnologies.

SUBSTANCE: invention relates to the biotechnology. Described is a method of analyzing at least two or more analytes of multiple single cells, involving: (a) providing a plurality of connectivity retaining elements (CE), where each CE includes a unit cell; (b) carrying out lysis of single cells in CE, wherein analyzed substances in a single cell are released in CE; (c) providing a first reporter group for the first analyte in a single cell in each CE; (d) providing a second reporter group for a second analyte in a single cell in each CE; (e) modification of analyzed substances, as a result of which at least part of the first and second analyzed substances contained in CE, includes first and second reporter groups, respectively; (f) combining CE, comprising analytes containing reporter groups; (g) compartmentalisation of CE, comprising first and second analytes, including first and second reporter groups, respectively, to form multiple compartments; (h) providing a third reporter group for the first analyte, comprising a first reporter group, in each CE; (i) providing a fourth reporter group for a second analyte, comprising a second reporter group, in each CE; (j) additional modification of analyzed substances, as a result of which at least part of first analyzed substances includes first and third reporter groups, and at least part of second analyzed substances includes second and fourth reporter groups; (k) analysis of analyzed substances, including reporter groups, in each compartment, wherein in the conducted analysis, a single cell is detected, which is a source of each analyzed substance.

EFFECT: invention discloses a method and a composition for analyzing cellular components.

19 cl, 10 dwg, 2 ex