FIELD: biotechnology.
SUBSTANCE: described is a method for detecting cDNA of SARS-CoV-2 virus. Use of specific primers allows detecting genetic material of SARS-CoV-2 virus in analyzed samples by polymerase chain reaction (PCR). One pair of primers is selected to orf1ab gene. Their sequences: SEQ ID NO: 1-5' cagtctgtaccgtctgcgg 3', SEQ ID NO: 2-5' cagtactagtgcctgtgccg 3'. Length of amplicon is 158 base pairs. Two pairs of primers are selected to gene N. Their sequences and length of amplicons: SEQ ID NO: 3-5' ggtggaccctcagattcaactgg 3', SEQ ID NO: 4-5' ttttaccgtcaccaccacgaa 3', PCR product length is 250 base pairs; SEQ ID NO: 5-5' cgcattggcatggaagtcac 3', SEQ ID NO: 6-5' tgtctctgcggtaaggcttg 3', PCR product length is 203 base pairs. After PCR, reaction products are separated in electrophoresis with marker length. According to the presence and length of amplicons, the method enables detecting and identifying the presence of SARS-CoV-2 virus in biological material.
EFFECT: invention can find application in medicine in laboratory diagnostics COVID-19.
1 cl, 2 dwg, 4 tbl, 2 ex
| Title | Year | Author | Number |
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| SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 | 2021 |
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| OLIGONUCLEOTIDES FOR DETECTION OF SARS-CoV-2 MUTATION S:L452R | 2022 |
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