UNIVERSAL SYSTEM FOR SAMPLE PREPARING AND APPLICATION IN INTEGRATED SYSTEM OF ANALYSIS Russian patent published in 2015 - IPC C12M1/18 C12M1/42 B01J19/00 C12Q1/68 G01N1/02 G01N27/74 G01N35/08 G01N35/10 

FIELD: medicine.

SUBSTANCE: invention relates to biotechnology. A system consists of the following elements: a) module for sample preparing, made with the ability to trap analyte in a non-microliquid volume on a trapping particle, responding to the magnetic field, and direction of the trapping particle bound with the analyte, responding to the magnetic field, through the first microliquid channel; b) reaction module, which includes a reaction chamber, which has liquid communication with the first microliquid channel and made with a possibility to immobilise the trapping particle bound with the analyte, responding to the magnetic field, and carrying out the reaction of amplification of a multitude of analyte STR-markers. The module for sample preparing and the reaction module are integrated into a disposable cartridge, which consists of: 1) at least one combination of liquid chambers, 2) a board with reagents or a cartridge with reagents and 3) one or more than one pneumatically activated MOVe-valve; c) an analysis module. The system is configured for trapping the analyte, for carrying out a chemical or biochemical reaction with the analyte and for carrying out the analysis of a reaction product for less than 4 hours. Transfer of flowing means, resistant to leaks, is realised due to the application of MOVe-valves in the said system, a possibility to reduce dimensions of the device for sample preparation appears.

EFFECT: application of the claimed system makes it possible to select target organisms from samples with a large amount of background admixtures, differentiate two different strains of bacteria, trap cells and toxins in an effective way, and considerably reduce the volume of the target sample.

30 cl, 104 dwg, 3 ex