Show metadata Hide metadata

(19)

(11)

2 768 753

(13)

(51)

IPC

G01N33/50(2006-01-01)

G01N33/569(2006-01-01)

C12Q1/686(2018-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2020125439, 2020-07-22

(24)

Start date

2020-07-22

(22)

Actual filing date

2020-07-22

(45)

Published

2022-03-24

(72)

Inventor

Nefedchenko Aleksej VasilevichGlotov Aleksandr GavrilovichGlotova Tatyana IvanovnaKoteneva Svetlana Vladimirovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauki Sibirskij Federalnyj Nauchnyj Tsentr Agrobiotekhnologij Rossijskoj Akademii Nauk Ran)

SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND PROBES FOR DETECTING BOVINE RESPIRATORY SYNCYTIAL INFECTION VIRUS AND BOVINE GAPDH GENE AND METHOD FOR DETECTING RNA OF RESPIRATORY SYNCYTIAL INFECTION VIRUS IN CATTLE Russian patent published in 2022 - IPC G01N33/50 G01N33/569 C12Q1/686 C12Q1/6876

Abstract RU 2768753 C2

FIELD: biotechnology.

SUBSTANCE: disclosed is a set of synthetic oligonucleotide primers and probes for detecting bovine respiratory syncytial infection virus (BRSV) and bovine GAPDH gene, characterized by that synthetic oligonucleotide primers and probes have nucleotide sequences: SEQ ID NO: 1–6. Also disclosed is a method for detecting RNA virus of respiratory syncytial infection in cattle using said kit.

EFFECT: group of inventions provides a highly specific and sensitive method based on a polymerase chain reaction (PCR) using real-time hybridisation-fluorescence detection, enabling detection and estimation of bovine BRSV virus concentration in biomaterial samples.

2 cl, 1 dwg, 3 tbl, 4 ex

Similar patents RU2768753C2

Title	Year	Author	Number
SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD OF BOVINE RESPIRATORY SYNCYTIAL INFECTION RNA VIRUS DETECTION BY SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION (PCR)	2009	Glotov Aleksandr Gavrilovich Glotova Tat'Jana Ivanovna Koteneva Svetlana Vladimirovna Nefedchenko Aleksej Vasil'Evich	RU2405039C1
METHOD OF DETECTING PATHOGENS OF RESPIRATORY INFECTIONS IN CATTLE: BPIV, BRSV, BHV-4, BCoV, BVDV-1, BVDV-2, BVDV-3 BASED ON MULTIPLEX POLYMERASE CHAIN REACTION (PCR)	2022	Glotov Aleksandr Gavrilovich Nefedchenko Aleksej Vasilevich Glotova Tatyana Ivanovna Koteneva Svetlana Vladimirovna	RU2798286C1
SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES AND METHOD FOR DETECTING BOVINE PESTIVIRUS H	2019	Nefedchenko Aleksej Vasilevich Glotov Aleksandr Gavrilovich Glotova Tatyana Ivanovna Koteneva Svetlana Vladimirovna	RU2728342C1
METHOD OF PRIMARY ISOLATION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS STRAINS, BOVINE RESPIRATORY SYNCITIAL VIRUS STRAIN FOR PREPARING DIAGNOSTIC PRODUCTS	2010	Glotova Tat'Jana Ivanovna Glotov Aleksandr Gavrilovich Stroganova Irina Jakovlevna	RU2451073C2
METHOD FOR DETECTION AND IDENTIFICATION OF CORONAVIRUSES IN CATTLE	2021	Nefedchenko Aleksej Vasilevich Koteneva Svetlana Vladimirovna Glotova Tatyana Ivanovna Glotov Aleksandr Gavrilovich	RU2766344C1
SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR DETECTING HERPES VIRUS OF CATTLE TYPE 4 (BHV-4) IN SAMPLES OF BIOMATERIAL	2018	Nefedchenko Aleksej Vasilevich Glotova Tatyana Ivanovna Glotov Aleksandr Gavrilovich Koteneva Svetlana Vladimirovna	RU2700750C1
METHOD FOR DETECTING GENOME OF PARAINFLUENZA VIRUS OF TYPE 3 IN BOVINE ANIMALS	2018	Chernykh Oleg Yurevich Sochnev Vasilij Vasilevich Bannov Vasilij Aleksandrovich Malyshev Denis Vladislavovich Drobin Yurij Dmitrievich Donnik Irina Mikhajlovna Ivanov Arkadij Vasilevich Lysenko Aleksandr Anatolevich Krivonos Roman Anatolevich Shevkoplyas Vladimir Nikolaevich Koshchaev Andrej Georgievich Kaloshkina Inna Muratovna Kulakova Anna Leonidovna	RU2696069C2
TEST SYSTEM FOR DETECTING DNA OF CAUSATIVE AGENT OF CONTAGIOUS CATTLE PLEUROPNEUMONIA BY REAL-TIME POLYMERASE CHAIN REACTION	2023	Biadovskaia Olga Petrovna Sprygin Aleksandr Vladimirovich Krotova Alena Olegovna Abed Alkhussen Mokhammad Shalina Kseniia Aleksandrovna Prutnikov Pavel Vladimirovich Fedorova Olga Evgenevna	RU2821042C1
OLIGONUCLEOTIDE PRIMERS FOR DETECTING BOVINE ENZOOTIC LEUKAEMIA VIRUS BY POLYMERASE CHAIN REACTION	2024	Agoltsov Valerii Aleksandrovich Padilo Larisa Pavlovna Sibgatullova Adylia Kamilevna Pochepnia Ekaterina Sergeevna Chernykh Oleg Iurevich Biriukova Oksana Petrovna Popova Olga Mikhailovna	RU2824666C1
SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING RNA ATYPICAL PESTIVIRUS OF CATTLE	2016	Koteneva Svetlana Vladimirovna Maksyutov Rinat Amirovich Glotov Aleksandr Gavrilovich Glotova Tatyana Ivanovna Sergeev Aleksandr Nikolaevich Agafonov Aleksandr Petrovich	RU2607025C1

RU 2 768 753 C2

Authors

Nefedchenko Aleksej Vasilevich

Glotov Aleksandr Gavrilovich

Glotova Tatyana Ivanovna

Koteneva Svetlana Vladimirovna

Dates

2022-03-24—Published

2020-07-22—Filed