FIELD: biotechnology; genetic engineering.
SUBSTANCE: method of detecting pathogens of respiratory infections in cattle is proposed: BPIV, BRSV, BHV-1, BHV-4, BCoV, BVDV-1, BVDV-2, BVDV-3 based on multiplex polymerase chain reaction (PCR). The proposed method includes the isolation of RNA/DNA from samples of biological material from cattle and a polymerase chain reaction with hybridization-fluorescence detection in real time for the simultaneous detection of viruses BVDV1, BVDV2, BVDV3, BRSV, BHV-1, BHV-4 BPI-3, BCoV causing respiratory disease in cattle. In this case, reverse transcription for cDNA synthesis is carried out directly during multiplex PCR, and the study of each sample is carried out in two independent reactions for the simultaneous detection of eight viruses in one sample.
EFFECT: method can be used in veterinary virology for the diagnosis of infectious diseases of farm animals, in particular, infectious rhinotracheitis, viral diarrhea — diseases of the mucous membranes, respiratory syncytial infection, coronavirus infection, herpes virus infection type 4, parainfluenza-3 of cattle.
1 cl, 5 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| TEST SYSTEM FOR DETECTING DNA OF CAUSATIVE AGENT OF CONTAGIOUS CATTLE PLEUROPNEUMONIA BY REAL-TIME POLYMERASE CHAIN REACTION | 2023 |
|
RU2821042C1 |
| SET OF REAGENTS FOR THE DETECTION OF PATHOGENS OF TICK-BORNE NEUROINFECTIONS | 2022 |
|
RU2800261C1 |
| KIT FOR DIFFERENTIATING CATTLE PESTIVIRUSES AND METHOD FOR DIFFERENTIATING CATTLE PESTIVIRUSES | 2019 |
|
RU2731716C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES AND METHOD FOR DETECTING BOVINE PESTIVIRUS H | 2019 |
|
RU2728342C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND PROBES FOR DETECTING BOVINE RESPIRATORY SYNCYTIAL INFECTION VIRUS AND BOVINE GAPDH GENE AND METHOD FOR DETECTING RNA OF RESPIRATORY SYNCYTIAL INFECTION VIRUS IN CATTLE | 2020 |
|
RU2768753C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR DETECTING HERPES VIRUS OF CATTLE TYPE 4 (BHV-4) IN SAMPLES OF BIOMATERIAL | 2018 |
|
RU2700750C1 |
| METHOD OF DIAGNOSING INVASIVE CANDIDIASIS AND SPECIES IDENTIFICATION OF ITS MAIN PATHOGENS USING PCR WITH HYBRIDIZATION-FLUORESCENT DETECTION IN REAL TIME | 2022 |
|
RU2809386C1 |
| TEST SYSTEM AND METHOD OF DETECTING SPECIFIC NUCLEIC ACID FRAGMENTS OF 16 PATHOGENS USING ISOTHERMAL AMPLIFICATION REACTION | 2023 |
|
RU2810751C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND A METHOD OF HIGHLY SENSITIVE DETECTION OF AFRICAN SWINE FEVER VIRUS DNA BY LOOP ISOTHERMAL AMPLIFICATION IN THE PRESENCE OF INTERNAL CONTROL SAMPLE DNA | 2022 |
|
RU2799410C1 |
| METHOD FOR MULTIPLEX IDENTIFICATION OF 32 FLAX GENETIC MARKERS | 2022 |
|
RU2804939C1 |
