FIELD: biotechnology.
SUBSTANCE: disclosed are synthetic oligonucleotide primers and probes and a method for detecting and identifying biological variants of bovine coronaviruses using synthetic oligonucleotide primers and probes based on polymerase chain reaction (PCR) using real-time hybridization-fluorescence detection. Disclosed method involves RNA extraction, reverse transcription, the study of each sample is carried out by multiplex PCR for the simultaneous detection of two virus variants BCoV and HCoV-OC43 by nucleocapsid N protein gene.
EFFECT: method has high sensitivity, specificity and efficiency in detecting and identifying BCoV and HCoV-OC43 in samples of biological material from animals.
2 cl, 1 tbl, 1 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTING PATHOGENS OF RESPIRATORY INFECTIONS IN CATTLE: BPIV, BRSV, BHV-4, BCoV, BVDV-1, BVDV-2, BVDV-3 BASED ON MULTIPLEX POLYMERASE CHAIN REACTION (PCR) | 2022 |
|
RU2798286C1 |
| TEST SYSTEM FOR DETECTING CORONAVIRUS INFECTION PATHOGEN GENOME IN CATTLE BY MEANS OF MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME | 2018 |
|
RU2694499C1 |
| REAGENT SET FOR DETECTING RNA OF SARS-COV-2 VIRUS, CAUSATIVE AGENT OF NEW CORONAVIRUS DISEASE COVID-2019, BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION IN REAL TIME | 2020 |
|
RU2732608C1 |
| METHOD FOR GENOME DETECTION OF CORONAVIRUS INFECTION CAUSATIVE AGENT IN CATTLE | 2018 |
|
RU2694558C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFICATION OF HUMAN CORONAVIRUS 2019-nCoV RNA BY PCR WITH HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2020 |
|
RU2734300C1 |
| SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 | 2021 |
|
RU2752902C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR 229E, NL63, OC43, HKU1 CORONAVIRUS RNA IDENTIFICATION BY FLUORESCENCE IN SITU HYBRIDISATION AND REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION | 2011 |
|
RU2473702C1 |
| SET OF OLIGODEOXYRIBONUCLETIDE PRIMERS AND FLUORESCENT-LABELED PROBE FOR IDENTIFICATION OF RNA OF HUMAN CORONAVIRUSES SARS AND 2019-NCOV BY RT-PCR METHOD WITH HYBRIDIZATION-FLUORESCENT DETECTION IN REAL TIME | 2020 |
|
RU2733665C1 |
| TEST SYSTEM FOR DETECTION OF SARS-CoV-2 OF OMICRON LINE BY SINGLE-STEP POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION | 2021 |
|
RU2772362C1 |
| REAGENT KIT FOR DETECTION OF SARS-COV-2 VIRUS RNA BY REAL-TIMR POLYMERASE CHAIN REACTION | 2022 |
|
RU2795939C2 |
