FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Presented is a method for differentiating three cattle pestiviruses (Pestivirus A, B and H) in a polymerase chain reaction. Disclosed method involves carrying out a polymerase chain reaction using hybridization-fluorescent detection in real time. Each sample is analyzed in two stages. At the first stage primers and probes are used common for all genus Pestivirus. At the second stage positive samples are analyzed in three independent reactions for differentiation of 3 species - Pestivirus A, B and H.
EFFECT: method can be used in veterinary science to detect possible contamination by pestiviruses of embryonic serums used for culturing cell cultures and producing biopreparations, cell cultures, as well as for diagnosis of infectious diseases of farm animals, particularly cattle pestiviruses.
2 cl, 4 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES AND METHOD FOR DETECTING BOVINE PESTIVIRUS H | 2019 |
|
RU2728342C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING RNA ATYPICAL PESTIVIRUS OF CATTLE | 2016 |
|
RU2607025C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND PROBES FOR DETECTING BOVINE RESPIRATORY SYNCYTIAL INFECTION VIRUS AND BOVINE GAPDH GENE AND METHOD FOR DETECTING RNA OF RESPIRATORY SYNCYTIAL INFECTION VIRUS IN CATTLE | 2020 |
|
RU2768753C2 |
| METHOD FOR DETECTION AND IDENTIFICATION OF CORONAVIRUSES IN CATTLE | 2021 |
|
RU2766344C1 |
| METHOD OF DETECTING PATHOGENS OF RESPIRATORY INFECTIONS IN CATTLE: BPIV, BRSV, BHV-4, BCoV, BVDV-1, BVDV-2, BVDV-3 BASED ON MULTIPLEX POLYMERASE CHAIN REACTION (PCR) | 2022 |
|
RU2798286C1 |
| METHOD FOR DIAGNOSIS OF CATTLE LEUKEMIA BY POLYMERASE CHAIN REACTION | 2018 |
|
RU2694617C1 |
| SET OF SEQUENCES OF PRIMERS AND ALLELE-SPECIFIC PROBES FOR DIFFERENTIATION OF KAPPA-CASEIN ALLELES IN CATTLE | 2020 |
|
RU2788734C2 |
| SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 | 2021 |
|
RU2752902C1 |
| METHOD OF DETECTING AND GENOTYPING BACTERIA Pasteurella multocida OF CATTLE OF SEROGROUPS A, B, D, E, F IN MULTIPLEX POLYMERASE CHAIN REACTION | 2014 |
|
RU2551962C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR DETECTING HERPES VIRUS OF CATTLE TYPE 4 (BHV-4) IN SAMPLES OF BIOMATERIAL | 2018 |
|
RU2700750C1 |
