FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is related to a medium for culturing cells without serum. Additives to the basic medium for increasing productivity are a composition of L-asparagine, succinic acid and fibroblast growth factor-2 (FGF-2) in different combinations, dextran sulphate and micro additives of nickel chloride, sodium molybdate, zinc chloride and iron citrate. Nutrient medium is the basic IMDM medium (Iskov medium - modification of Dulbecco medium) with known composition.
EFFECT: when growing cells in a given medium using said composition in different combinations, production of recombinant antibodies, viability and duration of cultivation is increased.
11 cl, 6 dwg, 3 ex
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