FIELD: biotechnology.
SUBSTANCE: described is a group of inventions involving using a composition as a lysing agent contained in whole blood cells, as an agent for stabilizing total RNA in whole blood and for RNA recovery by adsorption from a whole blood mixture with an adsorbent composition for nucleic acids and a method of extracting whole RNA from whole blood. In one embodiment, the composition in the form of an aqueous solution consists of guanidine thiocyanate, at least one buffer substance, at least one non-ionic detergent, ethylene diamine tetraacetate, and optionally proteinase and/or deoxyribonuclease and does not contain decomposition inhibitors and new synthesis of RNA in whole blood.
EFFECT: invention extends the range of products for cell lysis and RNA isolation from whole blood.
14 cl, 8 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD AND COMPOSITION FOR STABILISING CELL-FREE NUCLEIC ACIDS AND CELLS | 2018 |
|
RU2764245C2 |
| ENVIRONMENT AND DEVICE FOR COLLECTING, STORING AND TRANSPORTING BIOLOGICAL SAMPLES, AS WELL AS THEIR USE FOR THE STABILIZATION OF EXTRACELLULAR NUCLEIC ACIDS | 2022 |
|
RU2798013C1 |
| COMPOSITION AND METHOD FOR ISOLATION OF NUCLEIC ACIDS FROM COMPLEX MATERIAL USING ANTI-CHAOTROPIC SALT | 1999 |
|
RU2241004C2 |
| METHOD FOR PRESERVING BIOLOGICAL SAMPLE PROVIDING SAFETY OF NUCLEIC ACIDS | 2005 |
|
RU2322058C2 |
| METHOD FOR RNA AND DNA ISOLATION FROM DRY BIOLOGICAL SAMPLES STORED ON PAPER MEDIA AND SET FOR ITS IMPLEMENTATION | 2016 |
|
RU2628695C1 |
| METHOD FOR RECOVERY OF NUCLEIC ACIDS | 2014 |
|
RU2584346C2 |
| METHOD FOR ISOLATING OF COCCIDIOIDES IMMITIS DNA FOR POLYMERASE CHAIN REACTION (PCR) | 2005 |
|
RU2295569C1 |
| SORPTION COMPOSITE MEMBRANE AND BIOSEPARATING DEVICE FOR DNA EXTRACTION | 2016 |
|
RU2631934C1 |
| METHOD FOR DNA ISOLATION FROM PLANTS, SUITABLE FOR PCR | 2017 |
|
RU2672378C1 |
| METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS OUT OF URINE | 2006 |
|
RU2311640C1 |
