FIELD: biotechnology.
SUBSTANCE: group of inventions is described, including a method for RNA and DNA detection from dry biological samples and a set for RNA and DNA isolation from dry biological samples. The above method involves biological sample release from the carrier and convertion into a liquid form, cell walls lysis, precipitation, precipitate washing and nucleic acids elution therefrom. DNA and RNA precipitation is carried out with a solution containing isopropanol and C6H10O5 at initial concentration of 27 mg/ml, taken at a ratio of 40:0.5, respectively.
EFFECT: invention expands the arsenal of means for nucleic acids isolation.
9 cl, 5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR ISOLATING OF COCCIDIOIDES IMMITIS DNA FOR POLYMERASE CHAIN REACTION (PCR) | 2005 |
|
RU2295569C1 |
| METHOD FOR RECOVERY OF NUCLEIC ACIDS | 2014 |
|
RU2584346C2 |
| METHOD FOR DNA ISOLATION FROM PLANTS, SUITABLE FOR PCR | 2017 |
|
RU2672378C1 |
| METHOD FOR DNA RECOVERY FROM BONE MATERIAL | 2019 |
|
RU2724506C1 |
| METHOD OF PREPARING BIOLOGICAL MATERIAL FOR POLYMERASE CHAIN REACTION (PCR) GENE-AMPLIFICATING DIAGNOSIS | 1998 |
|
RU2134871C1 |
| METHOD FOR AUTOMATED EXTRACTION WITH SIMULTANEOUS PURIFICATION OF NUCLEIC ACIDS FROM SEVERAL BIOLOGICAL SAMPLES | 2014 |
|
RU2595374C2 |
| METHOD FOR EXTRACTION OF NUCLEIC ACIDS FROM NAIL PLATES | 2020 |
|
RU2751244C1 |
| UNIVERSAL METHOD OF DNA ISOLATION AND LYSIS MIXTURE FOR ITS IMPLEMENTATION | 2022 |
|
RU2807254C1 |
| METHOD FOR ISOLATION OF NUCLEIC ACIDS | 2004 |
|
RU2272072C1 |
| METHOD FOR ISOLATING RIBONUCLEIC ACIDS | 2002 |
|
RU2232810C1 |
