FIELD: biotechnology.
SUBSTANCE: invention relates to a method for producing aflibercept. Culturing mammalian cell expressing aflibercept in a temperature range of 35.0 to 38.0 °C. When the final cell density reaches 1x106 cells/ml to 1x108 cells/ml, culturing cells without transfer into another bioreactor is continued at low temperature from 30.0 to 35.0 °C.
EFFECT: invention allows achieving significant increase in the expression level of aflibercept with reduced number of aggregates and increases cell viability.
11 cl, 11 dwg, 1 tbl, 3 ex
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