FIELD: biotechnology; medicine.
SUBSTANCE: invention refers to biotechnology and medicine, namely to diagnosing infectious diseases, particularly to detecting genetic markers of coronavirus SARS-CoV-2. Kit for detection of coronavirus type SARS-CoV-2 by RT-PCR in real time includes oligonucleotide primers, which limit a fragment of RNA gene of dependent RNA polymerase, and a fluorescent labeled fluorescent (fluorescent probe) oligonucleotide having the following structure: CoV2-f (SEQ ID NO.1) tca caa ttc aga agt agg acc tg, CoV2-r (SEQ ID NO.2) agc ctc caa agg caa tag tgc, CoV2-prc (SEQ ID NO.3) R6G - tct tgc cga ata cca taa tga atc tgg caa - BHQ1.
EFFECT: present kit for detecting coronavirus type SARS-CoV-2 by RT-PCR in real time enables extending the range of available diagnostic agents.
1 cl, 1 dwg, 4 tbl
| Title | Year | Author | Number |
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| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
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RU2816270C2 |
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|
RU2744187C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELED PROBES FOR THE IDENTIFICATION OF HUMAN CORONAVIRUS SARS-COV-2 RNA BY ISOTHERMAL PCR WITH REAL-TIME HYBRIDIZATION-FLUORESCENCE DETECTION | 2021 |
|
RU2778855C1 |
| SET OF OLIGODEOXYRIBONUCLETIDE PRIMERS AND FLUORESCENT-LABELED PROBE FOR IDENTIFICATION OF RNA OF HUMAN CORONAVIRUSES SARS AND 2019-NCOV BY RT-PCR METHOD WITH HYBRIDIZATION-FLUORESCENT DETECTION IN REAL TIME | 2020 |
|
RU2733665C1 |
| TEST SYSTEM FOR DETECTION OF THE VIRUS GENOME OF PARAINFLUENZA 3 TYPES AT CATTLE WITH A MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME MODE | 2018 |
|
RU2681473C1 |
