METHOD FOR ASSESSING POSTVACCINAL IMMUNITY AGAINST BRUCELLOSIS Russian patent published in 2020 - IPC G01N33/53 

FIELD: medicine; immunology.

SUBSTANCE: invention can be used for evaluation of postvaccinal immunity against brucellosis in vitro. Method involves determining the concentration of spontaneous and antigen-induced interferon gamma in a culture supernatant in an enzyme immunoassay with subsequent calculation of the stimulation index. Inductor used is inactivated water-soluble brucellous antigen in final protein dilution of 50 mcg/ml, obtained from vaccine strain Brucella abortus 19 VA. Determination of the spontaneous and antigen-induced interferon gamma concentration in the culture supernatant is carried out spectrophotometrically at wavelength of 450 nm, wherein the level of specific cell response is assessed by a stimulation index taking into account the concentration of the interferon gamma-induced antigen in the culture supernatant. Vaccination criteria are defined: limits of values of stimulation index to 1.3, concentration of antigen of induced interferon gamma to 23 pg/ml and criteria of revaccination: limits of values of stimulation index to 2.6 and concentration of antigen of induced interferon gamma to 47.0 pg/ml, if the stimulation index exceeds 2.6 and the induced interferon gamma antigen is higher than 47.0 pg/ml, revaccination is contraindicated.

EFFECT: using the given method enables judging on the level of body sensibilization to a brucellous antigen by the strength of a specific cell response, preventing postvaccinal complications; in connection with which the method can be used before vaccination and revaccination from brucellosis and to establish the fact of sensibilization to the brucellous antigen as an allergotest in vitro.

1 cl, 3 ex, 5 tbl