METHODS FOR PRODUCING ALLOGENIC T-CELLS WITH CAR Russian patent published in 2024 - IPC C12N5/00 C12N5/783 A61K35/17 A61P35/00 

FIELD: biotechnology.

SUBSTANCE: disclosed are: a cell growth medium for T-cell propagation, comprising: first stimulator and second cell proliferation stimulator, each independently selected from a group consisting of IL-7 and IL-15, and an extracellular modulator of cell metabolism, which is extracellular potassium, in a concentration of not less than about 10 mM, where the first stimulant and the second stimulator are present at a concentration ratio of about 250:1 to about 10:1 IU/ml, and methods of producing a population of T-cells in vitro. Also disclosed is a cell growth medium for T-cell propagation, comprising: a first stimulator and a second cell proliferation stimulator, each of which is independently selected from the group consisting of IL-7 and IL-15; and optionally an extracellular modulator of cell metabolism, which is extracellular potassium, in a concentration of not less than about 10 mM, where the first stimulant and the second stimulator are present at a concentration ratio of about 250:1 to about 10:1, and wherein the concentration is in IU/ml.

EFFECT: invention can be used to obtain a population of T-cells.

31 cl, 5 dwg, 2 tbl, 4 ex