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2 743 586

(13)

(51)

IPC

C12Q1/68(2006-01-01)

(21) (22)

Application

2020118092, 2020-04-30

(24)

Start date

2020-04-30

(22)

Actual filing date

2020-04-30

(45)

Published

2021-02-20

(72)

Inventor

Krivoj Andrej AnatolevichCheranev Valerij VladimirovichKorostin Dmitrij Olegovich

(73)

Holder

Federalnoe Gosudarstvennoe Avtonomnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Natsionalnyj Issledovatelskij Meditsinskij Universitet Imeni N.I. Pirogova" Ministerstva Zdravookhraneniya Rossijskoj Federatsii Vo Rnimu Im. N.I. Pirogova Minzdrava Rossii)

METHOD OF DETERMINING EFFICIENCY OF ENRICHMENT OF NGS LIBRARY WITH EXON SECTIONS FOR SEQUENCING HUMAN EXOME Russian patent published in 2021 - IPC C12Q1/68

Abstract RU 2743586 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and can be used to check the quality of samples prepared for high-throughput DNA sequencing. To determine efficiency of NGS-library enrichment with exon sections for human exome sequencing qPCR with human DNA of the same NGS-library is performed before and after enrichment procedure. Each PCR reaction is carried out with at least 50 pg DNA of the NGS library. Efficiency of the qPCR reaction and the threshold cycles of the fluorescent signal of the NGS-library samples before and after the enrichment procedure are determined. Enrichment efficiency of the NGS library is then calculated using the given formula.

EFFECT: technical result consists in improvement of NGS-library enrichment efficiency evaluation accuracy.

1 cl, 2 ex, 4 dwg

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RU 2 743 586 C1

Authors

Krivoj Andrej Anatolevich

Cheranev Valerij Vladimirovich

Korostin Dmitrij Olegovich

Dates

2021-02-20—Published

2020-04-30—Filed