FIELD: molecular biology, biotechnology, forensic science and forensics, genetic research.
SUBSTANCE: a method for obtaining molecular autosomal human STR markers for identifying an unknown individual; and a set of synthetic oligonucleotide primers for conducting multiplex polymerase chain reaction (PCR). The genotyping kit includes a composition formed by 34 pairs of synthetic original oligonucleotide primers for loci in the human genome, including selected 34 autosomal STR markers, which allows multiplex PCR with primer sequences SEQ ID NO: 1 - SEQ ID NO: 68. Synthetic oligonucleotide primers are chosen to successfully amplify selected loci in a single multiplex reaction and subsequent sequencing using the Massive Parallel Sequencing (NGS) method. A method for identifying an unknown individual is presented.
EFFECT: can be used for DNA testing to identify an unknown individual and determine biological relationship in the Russian population with high sensitivity, specificity and accuracy compared to existing analogues.
6 cl, 1 tbl, 1 ex
