FIELD: medicine; biology; biotechnology.
SUBSTANCE: invention relates to clinical and laboratory diagnostics, molecular biology, genomics and genetics. Described is a method of enriching a library of DNA fragments with protein-coding sequences (exome) for subsequent sequencing by high-throughput second-generation sequencing (NGS), characterized by that for selection of protein-coding regions using biotinylated in vitro transcripts obtained from pre-performed full exome enrichment.
EFFECT: invention extends the range of methods for producing a library of DNA fragments for full exome sequencing.
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