METHOD FOR DETERMINING THE AMOUNT OF SESQUITERPENIC ACIDS IN TERMS OF VALERENIC ACID BY HPLC IN PHARMACEUTICALS Russian patent published in 2021 - IPC A61K36/84 A61K9/20 A61K9/06 G01N1/28 G01N1/38 G01N30/06 G01N30/38 

FIELD: pharmaceuticals +.

SUBSTANCE: invention relates to a method for the quantitative determination of the amount of sesquiterpenic acids in the drug "Valerian tincture" in the drug form "tincture", or in the drug "Valerian extract" in the drug form "film-coated tablets", or in the pharmaceutical substance "Valerian extract thick" by high-performance liquid chromatography (HPLC), including the use of ethyl alcohol 50% as a solvent for the preparation of solutions of the test sample of the drug "Valerian extract" in the dosage form "film-coated tablets" and the pharmaceutical substance "Valerian extract thick", columns for HPLC with octadecylsilyl silica gel sorbent, gradient elution method with two mobile phases: acetonitrile and phosphoric acid with a concentrated solution of 5.0 g / L in water, spectrophotometric detection at a wavelength of 220 nm, use of valerenic acid as a standard sample.

EFFECT: method allows quantitative determination of active substances in the drug "Valerian tincture" in the drug form "tincture", or in the drug "Valerian extract" in the drug form "film-coated tablets", or in the pharmaceutical substance "Valerian extract thick".

1 cl, 3 tbl, 5 dwg