FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. A method for producing a recombinant polypeptide includes: providing a fueled periodic bioreactor with a volume of 100 L to 25,000 L containing cells capable of expressing the recombinant polypeptide asphotase alpha, and a culture medium suitable for such expression, with the culture medium containing from about 25 microns to about 300 microns of zinc; cultivation of these cells under conditions suitable for the expression of recombinant asphotase alpha, while adding at least one bolus of fed batch to the culture medium during cultivation and culturing the cells at the first temperature until a cell density of at least approximately 2.5x106 viable cells is reached and the transition to a second temperature that is lower than the first temperature.
EFFECT: invention makes it possible to obtain a recombinant polypeptide with a high degree of efficiency.
21 cl, 53 dwg, 33 tbl, 10 ex
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