FIELD: biotechnology.
SUBSTANCE: following is described: a recombinant polypeptide obtained in a mammalian cell culture containing asphotase alpha (SEQ ID NO: 1) and having an average total sialic acid content of 0.9–3.5 mol sialic acid/mol protein monomer and another characteristic selected from the group consisting of the following: isoelectric focusing between 5.2 and 6.7, large glycan structure, as shown either on FIG. 41 or FIG. 42 is a 2-AB labeled oligosaccharide chromatogram profile as shown either on FIG. 38 or FIG. 39, MALDI-ToF fingerprint profile of the glycopeptide shown either on FIG. 40 or FIG. 44–49 and others. Also the following is provided: a pharmaceutical composition for treating a subject suffering from a condition associated with alkaline phosphatase deficiency containing the said recombinant polypeptide.
EFFECT: obtaining polypeptide with a high degree of efficiency.
25 cl, 53 dwg, 33 tbl, 10 ex
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