METHOD FOR QUANTITATIVE DETERMINATION OF AMOUNT OF PHENYLPROPANOIDS IN LILAC FLOWERS Russian patent published in 2021 - IPC A61K36/63 G01N21/00 

FIELD: chemical industry; pharmaceutical industry.

SUBSTANCE: invention relates to the chemical industry and the pharmaceutical industry, and in particular to a method for the quantitative determination of the amount of phenylpopanoids in flowers of common lilac (Syringa vulgaris L.). Disclosed is a method for the quantitative determination of phenylpropanoids, which consists in the preliminary obtaining of an aqueous-alcoholic extract from plant raw materials by extraction of 1 g of an exact weighed portion of a plant material crushed to a particle size of 1 mm with 60% ethyl alcohol for 45 minutes, in terms of a substance of a phenylpropanoic nature, by the method of direct spectrophotometry in the ratio of "raw material-extractant" - 1: 100, the determination of phenylpropanoids is carried out at a wavelength of 330 nm in terms of chlorogenic acid, the content of the sum of phenylpropanoids in terms of chlorogenic acid, and absolutely dry raw materials are calculated by the formula:

where x is the content of the sum of phenylpropanoids in terms of chlorogenic acid,%; D is the optical density of the test solution; D_o is the optical density of the solution of the state standard sample of chlorogenic acid; m is the mass of raw materials, g; m_o is the mass of the state standard sample of chlorogenic acid, g; W is the loss in mass on drying,%; in the absence of a standard sample of chlorogenic acid, it is advisable to use the theoretical value of its specific absorption index, equal to 497:

where x is the content of the sum of phenylpropanoids in terms of chlorogenic acid,%; D is the optical density of the test solution; m is the mass of raw materials, g; 497 - specific absorption rate of the state standard sample of rutin at 330 nm; W - loss in mass on drying,%.

EFFECT: above method for quantifying the amount of phenylpropanoids in common lilac flowers is specific and accurate.

1 cl, 2 dwg, 4 tbl, 2 ex