FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for amplifying circular DNA, and in particular long-stranded circular DNA in a cell-free system. More specifically, the present invention relates to a method for amplifying circular DNA, which comprises mixing circular DNA having an origin of replication sequence (chromosome origin (oriC)) with a reaction solution containing a first group of enzymes, which catalyze circular DNA replication; a second group of enzymes that catalyze the maturation of the Okazaki fragment and synthesize two sister circular DNAs consisting of catenan; a third group of enzymes that catalyze the separation of two sister circular DNA; as well as a buffer, NTP, dNTP, a source of magnesium ion and a source of an alkali metal ion, to obtain a reaction mixture, which is then subjected to a reaction of interaction.
EFFECT: circular DNA amplification.
26 cl, 38 dwg, 6 tbl, 16 ex
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