FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for replicating or amplifying circular DNA. More specifically, the present invention relates to a method for suppressing the formation of a DNA multimer as a byproduct when circular DNA having an origin of replication sequence (chromosome origin (oriC)) has been replicated or amplified using the following groups of enzymes: (1) the first group of enzymes that catalyze the replication of circular DNA; (2) a second group of enzymes that catalyze the maturation of the Okazaki fragment and synthesize two sister circular DNAs consisting of catenan; and (3) a third group of enzymes that catalyze the separation of two sister circular DNAs. In addition, the present invention also relates to a method comprising introducing oriC into circular DNA using a transposon.
EFFECT: improvement of method for replicating or amplifying circular DNA.
14 cl, 22 dwg, 3 tbl, 10 ex
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