METHOD FOR PRODUCING ROOTSTOCK MATERIAL (L-2) IN VITRO Russian patent published in 2021 - IPC A01H4/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. The invention is a method for producing rootstock material (L-2) in vitro, grafting test-tube plants and planting single-node cuttings on an agar nutrient medium containing macro- and microelements and vitamins based on the prescription of the nutrient medium, Fe-chelate, agar-agar, sucrose, while a high concentration of salts is added to the nutrient medium (Chelate - Fe of 50 mg / l and Ca (NO₃)₂ of 1000 mg / l) and for plant activity the plants are transplanted onto a nutrient medium containing 1 mg of 6 BAP and 0.05 g / l of succinic acid, while the concentration of micro-salts: ZnSO₄ * 7H₂O - 4.3 mg / l and H₃BO₃ - 3.1 mg / l, the calcium source is also changed in the form (from CaCl₂ * 2H₂O to Ca (NO₃)₂ * 4H₂O), the transplant is carried out after 3 weeks, during reproduction, special attention is paid to the vitreousness or super-water content of tissues, while the optimal conditions for the cultivation of L-2 are: temperature of 22-26 degrees, illumination of 2500-5000 lux with a 16-hour photoperiod.

EFFECT: invention makes it possible to increase the yield of plants up to 70%.

1 cl, 3 tbl