METHOD FOR OBTAINING IMMOBILIZED ENZYME PREPARATION BASED ON TRYPSIN, HYALURONIC ACID AND POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS Russian patent published in 2021 - IPC A61K35/00 A61K38/46 A61K47/36 A61P17/02 C12N11/08 

FIELD: chemical and pharmaceutical industry; medicine.

SUBSTANCE: present invention relates to the field of chemical and pharmaceutical industry and medicine, namely, to a method for producing an immobilized enzyme preparation based on trypsin, polysaccharides modified with vinyl monomers, and hyaluronic acid, including the dissolution of trypsin in an aqueous solution of low molecular weight hyaluronic acid 300 kDa or medium molecular weight hyaluronic acid 500 kDa or high molecular weight hyaluronic acid 800 kDa in a ratio of 10 mg of trypsin per 2 ml of an aqueous solution of low molecular weight hyaluronic acid 300 kDa or medium molecular weight hyaluronic acid 500 kDa or high molecular weight hyaluronic acid 800 kDa at a concentration of 1.5%, while stirring is carried out until complete dissolution at room temperature; then trypsin is immobilized by adding to the resulting mixture of graft copolymer carboxymethylcellulose 50-100 kDa (CMC) or chitosan 50-100 kDa (ChTS) with N-vinylimidazole (VI) or Ν, Ν-dimethylaminoethyl methacrylate (DMAEMA) in an amount from 100 to 290 mg for obtaining a liquid preparation or 300 to 500 mg for obtaining a gel. The present invention provides the development of a method for producing an immobilized liquid or gel enzyme preparation based on trypsin, polysaccharides modified with vinyl monomers, and hyaluronic acid of low molecular weight (300 kDa), medium molecular weight (500 kDa) or high molecular weight (800 kDa), whereby trypsin solutions can be stored at temperatures from 4 to 25°С for 21 days, and the enzyme becomes more stable in comparison with the native one and is able to penetrate into the deep layers of the skin, while the introduction of modified polysaccharides into the composition of the preparation not only makes it possible to vary the viscosity and consistency of the samples (from a liquid state to a gel with different fluidity), but also contributes to the formation conjugates with an enzyme with a large number of bonds and interactions between the polysaccharide and the enzyme compared to unmodified carboxymethylcellulose and chitosan, which further increases the stability of the drug and the concentration of the active substance in the affected area of the skin, and also provides a controlled (portioned) release of trypsin, maintaining its required concentration for a long time.

EFFECT: invention is aimed at increasing penetrating ability and stability of active ingredients.

1 cl, 3 dwg, 1 ex