METHOD FOR PRODUCING FICIN PREPARATION IN GEL BASED ON CARBOXYMETHYLCELLULOSE Russian patent published in 2022 - IPC C12N11/04 C12N11/12 A61K38/46 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. A method is proposed for obtaining a heterogeneous preparation in a gel based on ficin and carboxymethyl cellulose, including the immobilization of an enzyme preparation with an enzyme concentration of 3 mg/ml solution in 0.05 Μ borate buffer with 0.1 Μ KCl in the composition, pH 9.0 in a ratio of 20 ml of the enzyme solution per 1 g of sodium salt carboxymethyl cellulose with a molecular weight of ~ 90 kDa; incubation at room temperature with occasional stirring, and washing with 0.05 Μ Tris-HCl buffer pH 7.5 until there is no protein in the washings.

EFFECT: invention provides an extension of the arsenal of carriers for obtaining immobilized ficin, which has greater activity and provides enzymatic reactions in solution and on a solid substrate.

1 cl, 3 dwg, 1 ex