METHOD FOR PRODUCING IMMOBILIZED ENZYME PREPARATION BASED ON BROMELAIN, HYALURONIC ACID AND POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS Russian patent published in 2021 - IPC A61K38/00 A61K38/56 A61K47/36 C12N11/08 A61P17/02 

Abstract RU 2750377 C1

FIELD: chemical and pharmaceutical industry; medicine.

SUBSTANCE: present invention relates to the field of chemical and pharmaceutical industry and medicine, namely, to a method for producing an immobilized enzyme preparation based on bromelain, polysaccharides modified with vinyl monomers, and hyaluronic acid. The method includes the dissolution of bromelain in an aqueous solution of low-molecular-weight hyaluronic acid 300 kDa or medium-molecular-weight hyaluronic acid 500 kDa or high-molecular-weight hyaluronic acid 800 kDa in a ratio of 10 mg of bromelain per 2 ml of an aqueous solution of low-molecular-weight hyaluronic acid 300 kDa or medium-molecular-weight hyaluronic acid 500 kDa or high-molecular-weight hyaluronic acid 800 kDa at a concentration of 1.5%. Mixing is carried out until complete dissolving at room temperature. Bromelain is then immobilized by adding to the resulting mixture of a graft copolymer of carboxymethylcellulose 50-100 kDa (CMC) or chitosan 50-100 kDa (CHTS) with N-vinylimidazole (VI) or N,N-dimethylaminoethylmethacrylate (DMAEMA) in an amount of 100 to 290 mg to obtain a liquid preparation or 300 to 500 mg to obtain a gel. Due to the invention, bromelain solutions can be stored at temperatures from 4 to 25ºC for 21 days, and the enzyme becomes more stable in comparison with the native one and it is able to penetrate into the deep layers of the skin. The introduction of modified polysaccharides into the composition of the preparation allows not only to vary the viscosity and consistency of the samples (from the liquid state to the gel with different fluidity), but also promotes the formation of conjugates with an enzyme with a large number of bonds and interactions between the polysaccharide and the enzyme, compared with unmodified carboxymethylcellulose and chitosan. This further increases the stability of the preparation and the concentration of the active substance in the affected area of the skin, and provides a controlled (portion) release of bromelain, maintaining its necessary concentration for a long time.

EFFECT: increased stability of the preparation.

1 cl, 3 dwg, 1 ex

Similar patents RU2750377C1

Title Year Author Number
METHOD FOR OBTAINING IMMOBILIZED ENZYME PREPARATION BASED ON TRYPSIN, HYALURONIC ACID AND POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS 2020
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Pankova Svetlana Mikhajlovna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
  • Pavlovets Vyacheslav Viktorovich
RU2750376C1
METHOD FOR PRODUCING IMMOBILIZED ENZYME PREPARATION BASED ON COLLAGENASE, HYALURONIC ACID AND POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS 2020
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Belyaeva Tatyana Nikolaevna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
  • Koroleva Viktoriya Aleksandrovna
  • Pavlovets Vyacheslav Viktorovich
RU2750382C1
METHOD FOR PRODUCING IMMOBILIZED ENZYME PREPARATION BASED ON PAPAIN, POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS 2020
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
  • Koroleva Viktoriya Aleksandrovna
  • Pavlovets Vyacheslav Viktorovich
RU2750378C1
METHOD FOR OBTAINING AN IMMOBILIZED ENZYME PRODUCT BASED ON FICIN, HYALURONIC ACID AND POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS 2020
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Belyaeva Tatyana Nikolaevna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
  • Koroleva Viktoriya Aleksandrovna
  • Pavlovets Vyacheslav Viktorovich
RU2744457C1
METHOD FOR PRODUCING HYBRID PREPARATION OF BROMELAIN AND CARBOXYMETHYLCELLULOSE IN THE FORM OF A DENSE SOLUTION 2022
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Olshannikova Svetlana Sergeevna
  • Redko Yuliya Aleksandrovna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
RU2788454C1
METHOD FOR OBTAINING COMPOSITE PREPARATION OF BROMELAIN AND SODIUM ALGINATE IN THE FORM OF A DENSE SOLUTION 2022
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Olshannikova Svetlana Sergeevna
  • Malykhina Natalya Viktorovna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
RU2792785C1
METHOD FOR PRODUCING FICIN PREPARATION IN GEL BASED ON CARBOXYMETHYLCELLULOSE 2021
  • Kholyavka Marina Gennadevna
  • Olshannikova Svetlana Sergeevna
  • Artyukhov Valerij Grigorevich
RU2771183C1
METHOD OF PRODUCING A HYBRID PREPARATION OF BROMELAIN AND CHITOSAN ASCORBATE IN FORM OF A THICK SOLUTION 2023
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Goncharova Svetlana Sergeevna
  • Redko Yuliya Aleksandrovna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
RU2819793C1
METHOD FOR OBTAINING HYBRID PREPARATION OF PAPAIN AND CARBOXYMETHYLCELLULOSE IN THE FORM OF A DENSE SOLUTION 2022
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Olshannikova Svetlana Sergeevna
  • Redko Yuliya Aleksandrovna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
RU2795425C1
METHOD FOR OBTAINING FICIN AND CHITOSAN ACETATE HYBRID PREPARATION IN THE FORM OF A DENSE SOLUTION 2022
  • Kholyavka Marina Gennadevna
  • Artyukhov Valerij Grigorevich
  • Pankova Svetlana Mikhajlovna
  • Olshannikova Svetlana Sergeevna
  • Malykhina Natalya Viktorovna
  • Lavlinskaya Mariya Sergeevna
  • Sorokin Andrej Viktorovich
RU2792783C1

RU 2 750 377 C1

Authors

Kholyavka Marina Gennadevna

Artyukhov Valerij Grigorevich

Pankova Svetlana Mikhajlovna

Lavlinskaya Mariya Sergeevna

Sorokin Andrej Viktorovich

Koroleva Viktoriya Aleksandrovna

Pavlovets Vyacheslav Viktorovich

Dates

2021-06-28Published

2020-07-27Filed