METHOD FOR OBTAINING AN IMMOBILIZED ENZYME PRODUCT BASED ON FICIN, HYALURONIC ACID AND POLYSACCHARIDES MODIFIED WITH VINYL MONOMERS Russian patent published in 2021 - IPC A61K38/00 A61K38/46 A61K47/36 C12N11/08 A61P17/02 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely, to a method for obtaining an immobilized enzyme product based on ficin, polysaccharides modified with vinyl monomers, hyaluronic acid, ficin dissolving in an aqueous solution of low molecular weight hyaluronic acid 300 kDa, or medium molecular weight hyaluronic acid 500 kDa, or high molecular weight hyaluronic acid 800 kDa in a ratio of 10 mg ficin per 2 ml of an aqueous solution of low molecular weight hyaluronic acid 300 kDa, or medium molecular weight hyaluronic acid 500 kDa, or high molecular weight hyaluronic acid of 800 kDa at a concentration of 1,5%, while mixing is carried out until complete dissolution at room temperature; then ficin is immobilized by adding to the resulting mixture of carboxymethyl cellulose graft copolymer with a molecular weight of 50-100 kDa (CMC) or chitosan (CTS) with a molecular weight of 50-100 kDa with N-vinyl imidazole (VI) or N,N-dimethylaminoethyl methacrylate (DMAEMA) in an amount of 100 to 290 mg to obtain a liquid product or from 300 to 500 mg to obtain a gel. Present invention provides the development of a method for obtaining an immobilized liquid or gel-like enzyme product based on ficin, polysaccharides modified with vinyl monomers, and hyaluronic acid of low molecular weight 300 kDa, medium molecular weight 500 kDa or high molecular weight 800 kDa, due to which solutions of ficin can be stored at temperatures from 4 to 25°C from within 21 days, while the enzyme becomes more stable in comparison with the native one and is able to penetrate into the deep layers of the skin; at the same time, the introduction of modified polysaccharides into the composition of the drug allows not only to vary the viscosity and consistency of the samples (from a liquid state to a gel with different fluidity), but also promotes the formation of conjugates with an enzyme with a large number of bonds and interactions between the polysaccharide and the enzyme, compared to unmodified carboxymethylcellulose and chitosan, which further increases the stability of the drug and the concentration of the active substance in the affected area of ​​the skin, and also provides a controlled (portioned) release of ficin, maintaining its required concentration for a long time, in addition, the ability of modified polysaccharides to form stable films protects the enzyme and the treated area of ​​the skin from drying out, makes it easy to remove the drug along with pus and exudate.

EFFECT: provides the development of a method for obtaining an immobilized liquid or gel-like enzyme product based on ficin.

1 cl, 3 dwg, 1 ex