METHOD FOR MODELING INHALATION DAMAGE BY SULFURY IPRIT Russian patent published in 2021 - IPC G09B23/28 

FIELD: experimental medicine.

SUBSTANCE: invention relates to experimental medicine, in particular to military toxicology, and can be used for research purposes to simulate inhalation damage by sulfur mustard gas. The method involves poisoning small laboratory animals under anesthesia with an aerosol of a solution obtained by dissolving sulfur mustard in a 4% aqueous solution of dimethyl sulfoxide, with a concentration of sulfur mustard 1.37 mg/ml by introducing into the distal parts of the bronchopulmonary system to each rat participating in the experiment using microsprayer in the form of an aerosol with a dispersion of 32.1±2.6 microns. A solution of sulfur mustard gas in a volume of 100 mcl per 100 g of rat body weight is placed in a microspray syringe and administered to the rat intratracheally through the closing glottis. The microsprayer penetration is monitored by visual control of respiratory excursions when the microsprayer piston moves towards itself, and mechanical pressure is applied to the piston until the microsprayer is completely emptied. The rats are taken out of the experiment on days 1, 3, 7, 10 and 14 from the beginning of their poisoning with an aerosol of a solution of sulfur mustard with an absorbed dose of sulfur mustard of 1.37 mg/kg of rat body weight, 6 rats per day by decapitation.

EFFECT: invention allows obtaining repeatable models of local inhalation lesions in all poisoned rats, which reliably reflect the effect of sulfur mustard gas, by which, during necropsy, it is possible to visually assess the condition of the left lung of rats at each observation period and perform a pathomorphological study of micropreparations.

2 cl, 2 dwg, 3 ex