METHOD FOR OBTAINING HYPERIMMUNE RABBIT BLOOD SERUM TO YERSINIA PSEUDOTUBERCULOSIS DIMETHYL SULFOXIDE ANTIGEN USING HYDROXYAPATITE AS ADJUVANT Russian patent published in 2021 - IPC A61K35/16 A61K39/395 A61B10/00 A61P37/04 G01N33/49 

FIELD: pharmaceutical industry.

SUBSTANCE: invention relates to the pharmaceutical industry, namely to a method for producing hyperimmune rabbit blood serum to Yersinia pseudotuberculosis dimethyl sulfoxide antigen. The method of obtaining hyperimmune rabbit blood serum to Yersinia pseudotuberculosis dimethyl sulfoxide antigen consists in multiple immunization of the rabbit at least 5 times with a mixture of pseudotuberculosis microbe dimethyl sulfoxide antigen and hydroxyapatite, followed by taking blood from the immunized animal and separating serum from the immunized animal and separating immunization to a rabbit weighing 2-3 kg subcutaneously along the back at 4-5 points, 1 ml of a mixture of a solution of dimethyl sulfoxide antigen and 1% suspension of hydroxyapatite in a ratio of 1:1, in addition, a dose of dimethyl sulfoxide antigen Y. pseudotuberculosis for one immunization of a rabbit is 2 mg, the interval between subsequent immunizations is 2 weeks.

EFFECT: above method makes it possible to obtain hyperimmune rabbit blood serum with high content of specific antibodies to Y. pseudotuberculosis dimethyl sulfoxide antigen using HA as an adjuvant.

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