FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular, the invention provides a method for obtaining amplified methylome by elongating fragments and treating elongated fragments with methyltransferase and a source of methyl groups for the transformation of semi-methylated double-stranded DNA into fully methylated double-stranded DNA.
EFFECT: technical result is creation methods for DNA amplification to preserve methylation status.
61 cl, 7 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS FOR DISCRETE AMPLIFICATION OF COMPLETE GENOME | 2016 |
|
RU2736351C2 |
| GENOME-WIDE LIBRARIES OF INDIVIDUAL CELLS FOR BISULFITE SEQUENCING | 2018 |
|
RU2770879C2 |
| METHODS OF OBTAINING LIBRARIES OF TWO-CHAIN DNA AND METHODS OF SEQUENCING FOR IDENTIFYING METHYLATED CYTOSINES | 2015 |
|
RU2688485C2 |
| TRANSPOSITION WITH PRESERVATION OF GENE ADHESION | 2015 |
|
RU2709655C2 |
| ANALYSIS SYSTEM FOR ORTHOGONAL ACCESS TO BIOMOLECULES AND THEIR LABELLING IN CELL COMPARTMENTS | 2017 |
|
RU2771892C2 |
| TRANSPOSITION WITH PRESERVATION OF GENE ADHESION | 2015 |
|
RU2736728C2 |
| TAGMENTATION USING IMMOBILIZED TRANSPOSOMES WITH LINKERS | 2018 |
|
RU2783536C2 |
| METHOD FOR ANALYSING DISORDERS RELATED TO OVARIAN CARCINOMA | 2008 |
|
RU2511408C2 |
| HIGH-PERFORMANCE SINGLE CELL SEQUENCING WITH REDUCED AMPLIFICATION ERROR | 2019 |
|
RU2744175C1 |
| HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS | 2012 |
|
RU2603265C2 |
