FIELD: biotechnology.
SUBSTANCE: group of inventions is described, including a transposome complex for tagmentation, a composition for tagmentation, modified oligonucleotide for tagmentation, a method for the production of a library of labeled fragments of nucleic acids from double-stranded target nucleic acid, a method for the production of a transposome complex bound to solid substrate, a method for obtaining a sample for sequencing. In one of implementations, the transposome complex for tagmentation contains transposase, the first and the second transposons of DNA, and a cleaved linker.
EFFECT: invention expands the arsenal of means for tagmentation.
35 cl, 12 dwg, 7 tbl, 9 ex
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