METHOD FOR MICRO-DILUTION IN LIQUID MEDIUM FOR EVALUATING AND DETERMINING MINIMUM INHIBITORY CONCENTRATION OF ANTIBACTERIAL POLYPEPTIDES Russian patent published in 2021 - IPC A61K38/12 C12Q1/18 

Abstract RU 2754667 C2

FIELD: medicine.

SUBSTANCE: present invention relates to the field of medicine, namely, to a method for analyzing the sensitivity of gram-positive bacteria to an antibacterial peptide with broth micro-dilution (BMD) in liquid medium, including the evaluation of the specified antibacterial peptide in liquid medium with the addition of animal blood serum and a reducing agent, wherein the specified antibacterial peptide is polypeptide-lysine, and the evaluation of the specified antibacterial peptide includes the determination of the minimum inhibitory concentration (MIC) of the specified antibacterial peptide.

EFFECT: present invention provides an improved analysis by the method for broth micro-dilution (BMD) in a liquid medium, in which the transfer effect is eliminated.

14 cl, 12 dwg, 6 tbl, 1 ex

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RU 2 754 667 C2

Authors

Shukh, Rejmond

Dates

2021-09-06Published

2017-05-12Filed