METHOD FOR MODELING FOCAL ISCHEMIC STROKE OF BRAIN Russian patent published in 2021 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: invention relates to experimental medicine. A method for modeling focal ischemic stroke of the brain in rats includes the formation of an autologous blood clot and the subsequent introduction of the formed clot through a catheter into the internal carotid artery. At the same time, permanent ligation of brain vessels on one side is performed in the experimental animal under anesthesia: the common carotid, external carotid and pterygoid arteries. A catheter is inserted through an incision in the common carotid artery into the internal carotid artery at a distance of 14-18 mm from the place of bifurcation of the common carotid artery to the external and internal carotid arteries. 400 microliters of arterial blood are taken. A blood clot is formed by mixing arterial blood in a syringe with 50 mcl of a thromboplastin solution and 50 mcl of a 4% CaCl₂ solution. After 20 minutes, the formed clot with a volume of 100 mcl is injected through a catheter into the internal carotid artery. After 10 minutes, the catheter is removed and the surgical wound is sutured.

EFFECT: invention provides an increase in the relevance of the model of thromboembolic stroke, a high degree of reproducibility, obtaining pronounced structural and functional changes in the brain.

1 cl, 2 dwg