FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and is an application of psicose-6-phosphate phosphatase for the production of D-psicose, where psicose-6-phosphate phosphatase contains A motif, represented by Xa1-Xa2-Xa3-DPLDG-Xa4 or Xa1-Xa2-Xa3-DPIDG-Xa4, wherein Xa1 is W, F, V, I or A, Xa2 is I, F, V, A or absent, Xa3 is V, I or L, and Xa4 is T or S, and B motif, represented by Ya1-D-Ya2-Wa1-Ya3-Wa2-Ya4-Wa3, wherein Ya1 is W, Y, T, L or V, Ya2 is V, I, C, F or A, Wa1 is AAG, AAS, SAG, APG, APF, AGG, APL or AGA, Ya3 is W, I, P, M, V, Y, F, R, L, T or S, Wa2 is LLV, LIV, LLI, LII, ILI, FIA, ALV, IIA, VLV, VIL, TIG, NFC or PIF, Ya4 is E, R, S, T, L, K or P, and Wa3 is EAGG, EGGG, EAKG, KAGG, AAGG, YVDG, EAGA or RLGV.
EFFECT: invention makes it possible to increase the yield of D-psicose.
16 cl, 4 dwg, 1 tbl, 5 ex
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