GENETIC CONSTRUCT BASED ON CRISPR/CAS9 GENOME EDITING SYSTEM ENCODING CAS9 NUCLEASE FOR GENOME EDITING OF MONOCOTYLEDONOUS GRAIN CROPS BASED ON DOUBLE SELECTION OF PLANTS Russian patent published in 2021 - IPC C12N15/09 C12N15/63 C12N15/85 

Abstract RU 2762830 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, particularly, to a genetic construct based on the CRISPR/Cas9 genome editing system encoding the Cas9 nuclease, containing the bar gene-selectable marker and the gfp reporter gene.

EFFECT: invention ensures efficient selection of modified plants without additional molecular and biological testing.

1 cl, 6 dwg, 2 ex

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RU 2 762 830 C1

Authors

Shulga Olga Albertovna

Miroshnichenko Dmitrij Nikolaevich

Klementeva Anna Aleksandrovna

Timerbaev Vadim Rafailovich

Dolgov Sergej Vladimirovich

Dates

2021-12-23Published

2020-10-05Filed