FIELD: biotechnology.
SUBSTANCE: invention relates to the genome using the CRISPR-Cas9 system and the LigD protein. This system will allow to edit the genome of Pseudomonas putida bacteria, including for the synthesis of new substances with antibiotic activity.
EFFECT: invention discloses a new method for simplified editing of the genome of the bacterium Pseudomonas putida which, in particular, allows simultaneous editing of a large number of genomic targets.
13 cl, 2 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DNA PROTEASE CUTTING AGENT BASED ON Cas9 PROTEIN FROM DEFLUVIIMONAS SP. | 2018 |
|
RU2712492C1 |
| DNA POLYMER BASED ON Cas9 PROTEIN FROM BIOTECHNOLOGICALLY SIGNIFICANT BACTERIUM CLOSTRIDIUM CELLULOLYTICUM | 2018 |
|
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| USE OF CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA FOR MODIFYING GENOMIC DNA IN CELLS | 2019 |
|
RU2724470C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA | 2019 |
|
RU2722934C1 |
| METHOD FOR OBTAINING THE CRISPR/CAS RIBONUCLEOPROTEIN COMPLEX PREPARATION AND PREPARATION FOR DETECTION OF THE BLAVIM-2 (METAL-BETA-LACTAMASE CLASS B VIM-2) PSEUDOMONAS AERUGINOSA ANTIBIOTIC RESISTANCE GENE IN ULTRA-LOW CONCENTRATIONS | 2020 |
|
RU2745637C1 |
| NEW COMPACT TYPE II CAS9 NUCLEASE FROM ANOXYBACILLUS FLAVITHERMUS | 2020 |
|
RU2749307C1 |
| METHOD FOR DETECTING THE ANTIBIOTIC RESISTANCE GENE BLAVIM-2 (METAL-BETA-LACTAMASE CLASS B VIM-2) PSEUDOMONAS AERUGINOSA IN ULTRALOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2020 |
|
RU2734520C1 |
| SYSTEM FOR INCREASING EFFICIENCY OF MAMMALIAN GENOME EDITING THROUGH HOMOLOGOUS REPAIR | 2022 |
|
RU2808045C1 |
| TOOL FOR CUTTING DOUBLE-STRANDED DNA USING CAS12D PROTEIN FROM KATANOBACTERIA AND HYBRID RNA PRODUCED BY FUSION OF GUIDE CRISPR RNA AND SCOUT RNA | 2020 |
|
RU2771626C1 |
| SYSTEM FOR ATTRACTING PROTEIN TO DNA BREAK SITE TO INCREASE EFFICIENCY OF MAMMALIAN GENOME EDITING | 2022 |
|
RU2808601C1 |
