METHOD FOR INCREASING THE AUTOLOGOUS ANTITUMOUR ACTIVITY OF NEUTROPHILS IN VITRO Russian patent published in 2021 - IPC A61K38/00 C12N5/78 

FIELD: biotechnology.

SUBSTANCE: method for increasing the autologous antitumour activity of neutrophils in vitro in patients with oncological diseases includes isolation of cells from the venous blood, cultivation thereof in the conditions of a CO₂incubator at 37°C in a nutrient medium at a concentration of cells of 1∙106 cells/ml, the new characteristic is that the incubation is conducted for 4 hours in an environment additionally containing 0.5 M glutamic acid and 48.5 mM glyceraldehyde-3-phosphate, wherein a solution of arginine is introduced into the medium 2 hours after the beginning of incubation at a final concentration of 2M.

EFFECT: invention provides a possibility of increasing the autologous antitumour activity of neutrophils in oncological diseases by metabolic activation of neutrophils in vitro.

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